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Figure 3 | Microbial Cell Factories

Figure 3

From: Functional display of heterotetrameric human protein kinase CK2 on Escherichia coli: a novel tool for drug discovery

Figure 3

Detection of CK2 α and β subunit interaction on the bacterial surface. Outer membrane preparations of E. coli strains were precipitated with anti-CK2α antibody-loaded beads. The supernatant as well as the precipitate of each sample were separated on a SDS-Gel (10% acrylamide), stained with Coomassie brilliant blue (a) or analyzed by Western blotting using an anti-CK2-β serum for co-precipitation of CK2β-AT (b). OmpF and OmpA in the supernatant fraction served as an internal control for the concentration of outer membrane proteins.

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Microbial Cell Factories

ISSN: 1475-2859

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