Table 1 Summary of FMO2 truncation variants generated in the present study. The sequence of the reverse primer to amplify the truncated gene from the full-length template is shown.
From: Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis
FMO2 variant | Reverse primer |
|---|---|
FMO2*1X517 (FMO2Δ19) | 5′-TTA AGA AGC TTT TAC AAC AGA AAA GAA ACT GAG AAA TTA G-3′ |
FMO2*1X510 (FMO2Δ26) | 5′-TTA AGA AGC TTT TAA TTA GAT GAA TCC TTC AGG GC-3′ |
FMO2*1X505 (FMO2Δ31) | 5′-TTA AGA AGC TTT TAC AGG GCC CGA GTC-3′ |
FMO2*1X500 (FMO2Δ36) | 5´-TTA AGA AGC TTT TAG AGT GGC TTC AGT ATT C-3′ |
FMO2*1X496 (FMO2Δ40) | 5′-TTA AGA AGC TTT TAT ATT CTT TGT TTC TGG GTG AAG-3′ |
FMO2*1X491 (FMO2Δ45) | 5′-TTA AGA AGC TTT TAG GTG AAG ATG GCA TTT C-3′ |