Figure 2
From: Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis
Western blot analysis of full-length and truncated FMO2*1 in the membrane fraction of recombinant E. coli strains. 50 µg total protein of the membrane preparation of the empty vector control (pMS470, 1), FMO2*1 (2), FMO2*1X517 (3), FMO2*1X510 (4), FMO2*1X505 (5), FMO2*1X500 (6), FMO2*1X496 (7), FMO2*1X491 (8) were loaded, respectively. (Std.) PageRuler Prestained Protein Ladder (Thermo Fisher Scientific). The blot was probed with anti-hFMO2 antibody.