Table 1 Bacterial strains, plasmids used in this study
From: Synthetic sugar cassettes for the efficient production of flavonol glycosides in Escherichia coli
Vectors and plasmids | Description | Source/references |
|---|---|---|
pGEM®-T easy vector | General cloning vector, T7 and SP6 promoters, f1 ori, Ampr | Promega, USA |
pIBR181 | Multi mono-cistronic vector modified from pET28a + , f1 pBR322 ori, Kmr | Chaudhary et al. [13] |
piBR181-UGT78K1 | piBR181 vector carrying UGT78K1 | This study |
pET32(a)Â +Â ArGt-3 | pET32(a)Â +Â vector carrying ArGt-3 | This study |
pET-Duet-pgm2.galU | pET-Duet vector carrying pgm2.galU | This study |
piBR181-pgm2.galU.UGT78K1 | piBR181 vector carrying pgm2.galU.UGT78K1 | This study |
piBR181-glf.glk.pgm2.galU.UGT78K1 | piBR181 vector carrying glf.glk.pgm2.galU.UGT78K1 | This study |
pCDF-Duet-tgs.dh | pCDF-Duet vector carrying tgs.dh | Simkhada et al. [31] |
pACYC-Duet-ep.kr | pACYC-Duet vector carrying ep.kr | Simkhada et al. [31] |
piBR181-pgm2.galU | piBR181 vector carrying pgm2.galU | This study |
piBR181-tgs.dh.ep.kr | piBR181 vector carrying tgs.dh.ep.kr | This study |
PiBR181-tgs.dh.ep.kr.pgm2 | piBR181 vector carrying tgs.dh.ep.kr.pgm2.galU | This study |
piBR181-tgs.dh.ep.kr.pgm2. glf.glk | piBR181 vector carrying tgs.dh.ep.kr.pgm2.galU.glf.glk | This study |
piBR181-glf | piBR181 carrying glf from Zymomonas mobilis ZM4 | This study |
piBR181-glk | piBR181 carrying glk gene Zymomonas mobilis ZM4 | This study |
Strains | Description | Strain number | Source/references |
|---|---|---|---|
Escherichia coli XL-1 blue (MRF’) | General cloning host |  | Stratagene, USA |
E. coli BL21 (DE3) | ompT hsdT hsdS (rB-mB-) gal (DE3) | Â | Novagen |
E. coli BL21 (DE3)/piBR181-UGT78K1 | BL21(DE3) carrying piBR181-UGT78K1 from Glycin max (L.) Merr | S1 | This study |
E. coli BL21 (DE3) pET-Duet-pgm2.galU piBR181.UGT78K1 | BL21(DE3) carrying pET-Duet-pgm2.galU and piBR181-UGT78K1 | S2 | This study |
E. coli BL21 piBR181-pgm2.galU.UGT78K1 | BL21(DE3) carrying piBR181-pgm2.galU.UGT78K1 | S3 | This study |
E. coli BL21 piBR181-glf.glk.pgm2.galU.UGT78K1 | BL21(DE3) carrying piBR181-glf.glk.pgm2.galU.UGT78K1 | S4 | This study |
E. coli BL21 (DE3) pET32a-ArGt-3 | BL21 (DE3) pET32Â +Â vector carrying ArGt-3 from Arabidopsis thaliana | S5 | This study |
E. coli BL21 (DE3) pCDF-Duet-tgs.dh. pACYC-Duet-ep.kr. pET32a-ArGt-3 | BL21 (DE3) carrying pCDF-Duet-tgs.dh. pACYC-Duet-ep.kr. pET32a-ArGt-3 | S6 | This study |
E. coli BL21 (DE3) pCDF-Duet-tgs.dh. pACYC-Duet-ep.kr. piBR181.pgm2. pET32a-ArGt-3 | BL21 (DE3) carrying pCDF-Duet-tgs.dh. pACYC-Duet-ep.kr. piBR181.pgm2. pET32a-ArGt-3 | S7 | This study |
E. coli BL21 (DE3) piBR181.tgs.dh.ep.kr.pgm2. pET32a-ArGt-3 | BL21 (DE3) carrying piBR181.tgs.dh.ep.kr.pgm2. pET32a-ArGt-3 | S8 | This study |
E. coli BL21 (DE3) piBR181.tgs.dh.ep.kr.glf.glk.pgm2. pET32a-ArGt-3 | BL21 (DE3) carrying piBR181.tgs.dh.ep.kr.glf.glk.pgm2. pET32a-ArGt-3 | S9 | This study |