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Table 1 Expression of bgaB and gfp + under control of Pgrac01 and Pgrac100

From: Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli

  E. coli B. subtilis
IPTG concentration 0 mM 0 mM 0.001 mM 0.01 mM 0.1 mM
Pgrac01-bgaB      
0 h 7.7 ± 2.4 6.4 ± 1.4    
4 h 5.3 ± 1.1 38.9 ± 5.8 50 ± 12 53 ± 15 257 ± 31
Pgrac100-bgaB      
0 h 26 ± 9 75 ± 9    
4 h 37 ± 5.7 222 ± 68 343 ± 78 489 ± 119 817 ± 79
Pgrac01-gfp      
0 h 68 ± 4.7 5 ± 0.7    
4 h 47 ± 1.5 8 ± 0.5 7.0 ± 0.5 21 ± 0.6 190 ± 8
Pgrac100-gfp      
0 h 29 ± 0.7 73 ± 2.3    
4 h 37 ± 2.6 63 ± 2.6 106 ± 9.4 568 ± 117 1554 ± 65
  1. The data for BgaB and GFP activity presented have been obtained with pHT01-bgaB (Pgrac01-bgaB), pHT10-gfp + (Pgrac01-gfp) pHT212 (Pgrac212-bgaB) and pHT100-gfp (Pgrac100-gfp). BgaB activity is shown in Miller units while GFP indicated as activity is relative fluorescence unit (RFU). All experiments were carried out from at least three different colonies, and standard errors were calculated