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Fig 3 | Microbial Cell Factories

Fig 3

From: Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli

Fig 3

Expression of gfp + and bgaB fused to a His- or a Strep-tag. B. subtilis 1012 carrying (a) pHT253-bgaB (His-tag-BgaB), (b) pHT254-bgaB (BgaB-His-tag), (c) pHT255-bgaB (BgaB-Strep-tag), (d) pHT253-gfp (His-tag-GFP), (e) pHT254-gfp (GFP-His-tag), (f) pHT255-gfp (GFP-Strep-tag) were grown in LB medium to mid-log, and production of the recombinant proteins was induced by addition of 0.1 mM IPTG. Aliquots were taken before addition of IPTG and 2 and 4 h later. Cells were lysed by lysozyme, and aliquots corresponding to an OD600 of 0.13 were analyzed by SDS-PAGE (lane 0 h, before induction; lanes 2 h and 4 h, 2 and 4 h after induction). Black dots indicate the positions of BgaB or GFP, respectively

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