Fig 1From: Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli Map of the plasmid pHT254, different promoter sequences and the MCS. a, Map of the plasmid pHT254 with the backbone of pHT01 [16]; b, DNA sequences of the promoters present in Pgrac01 and Pgrac100, where the differences between these two promoters are underlined (UP element, −35, −15 regions) [11]; c, DNA sequence downstream of the RBS of plasmid pHT254, including the multi-cloning sites, the start codon, the His-tag and the stop codon (BamHI-Start codon-XbaI-AatII-His-tag-Stop codon/TAA-SmaI)Back to article page