Table 1 Bacterial strains and plasmids used in this study
Strains/plasmids | Relevant characteristics | Source/reference/notes |
|---|---|---|
Strains | Â | Â |
E. coli DH5α | F− endA1thi-1 recA1 relA1 gyrA96deoRΦ80dlacΔ(lacZ) M15 Δ(lacZYA-argF)U169hsdR17(rK −, mK +) λ− supE44 phoA | Invitrogen |
C. glutamicum RES167 | Restriction-deficient mutant of ATCC 13032, Δ(cglIM-cglIR-cglIIR) | University of Bielefeld |
Res167∆aroK | Res167 derivate, a fragment of DNA encoding for aroK was deleted | This study |
Res167∆aroK/pZB-aroG | Res167∆aroK derivate, containing plasmid pZB-aroG | This study |
Res167∆aroK/pZB-aroB | Res167∆aroK derivate, containing plasmid pZB-aroB | This study |
Res167∆aroK/pZB-aroD | Res167∆aroK derivate, containing plasmid pZB-aroD | This study |
Res167∆aroK/pZB-aroE | Res167∆aroK derivate, containing plasmid pZB-aroE | This study |
Plasmids | Â | Â |
pK18mobsacB | Mobilizable vector, for gene disruption in C. glutamicum | University of Bielefeld |
pK18mobsacB-aroK | Derived from pK18mobsacB, carrying aroK gene | This study |
pK18mobsacB-∆aroK | Derived from pK18mobsacB-aroK, a 573 bp fragment of aroK was deleted | This study |
pUC19-RiboJ | pUC19 carrying RiboJ | Sangon Biotech |
pACGFP | Plasmid carrying enhanced green fluorescence protein (GFP) gene | Invitrogen |
pXMJ19 | Shuttle vector (Camr, Ptac, lacIq, pBL1 oriV C.glu. pK18 oriV E. coli. ) | University of Bielefeld |
pXMJ19-RiboJ | pXMJ19 carrying RiboJ gene | This study |
pZB | Derived from pXMJ19, carrying both RiboJ and GFP genes | This study |
pZB-aroG | Derived from pZB, carrying aroG MU gene with various RBS | This study |
pZB-aroD | Derived from pZB, carrying aroD MU gene with various RBS | This study |
pZB-aroB | Derived from pZB, carrying aroB MU gene with various RBS | This study |
pZB-aroE | Derived from pZB, carrying aroE MU gene with various RBS | This study |
pXMJ19-aroG MU | pXMJ19 carrying aroG of which recognition sites of HindIII and PstI were mutated | This study |
pXMJ19-aroB MU | pXMJ19 carrying aroB of which recognition sites of BamHI and SpeI were mutated | This study |
pXMJ19-aroD MU | pXMJ19 carrying aroD of which recognition site of PstI were mutated | This study |
pXMJ19-aroE MU | pXMJ19 carrying aroE of which the recognition sites of EcoRI and SalI were mutated | This study |
pXMJ19-RiboJ-aroG MU-H | pXMJ19 carrying RiboJ and aroG MU gene with high strength RBS | This study |
pXMJ19-RiboJ-aroG MU-M | pXMJ19 carrying RiboJ and aroG MU gene with medium strength RBS | This study |
pXMJ19-RiboJ-aroG MU-L | pXMJ19 carrying RiboJ and aroG MU gene with low strength RBS | This study |
pXMJ19-RiboJ-aroB MU-H | pXMJ19 carrying RiboJ and aroB MU gene with high strength RBS | This study |
pXMJ19-RiboJ-aroB MU-M | pXMJ19 carrying RiboJ and aroB MU gene with medium strength RBS | This study |
pXMJ19-RiboJ-aroB MU-L | pXMJ19 carrying RiboJ and aroB MU gene with low strength RBS | This study |
pXMJ19-RiboJ-aroD MU-H | pXMJ19 carrying RiboJ and aroD MU gene with high strength RBS | This study |
pXMJ19-RiboJ-aroD MU-M | pXMJ19 carrying RiboJ and aroD MU gene with medium strength RBS | This study |
pXMJ19-RiboJ-aroD MU-L | pXMJ19 carrying RiboJ and aroD MU gene with low strength RBS | This study |
pXMJ19-RiboJ-aroE MU-H | pXMJ19 carrying RiboJ and aroE MU gene with high strength RBS | This study |
pXMJ19-RiboJ-aroE MU-M | pXMJ19 carrying RiboJ and aroE MU gene with medium strength RBS | This study |
pXMJ19-RiboJ-aroE MU-L | pXMJ19 carrying RiboJ and aroE MU gene with low strength RBS | This study |
pXMJ19-GHBH | Plasmid pXMJ19-RiboJ-aroGMU-H derivate, containing aroB-H module (Ptac-RiboJ-aroB, aroB gene with high strength RBS) | This study |
pXMJ19-GHBHDH | pXMJ19-GHBH derivate, containing aroD-H module (Ptac-RiboJ-aroD, aroD gene with high strength RBS) | This study |
plasmid-1 | pXMJ19-GHBHDH derivate, containing aroE-H module (Ptac-RiboJ-aroE, aroE gene with high strength RBS) | This study |
plasmid-2 | Plasmid pXMJ19-RiboJ-aroG MU-H derivate, containing aroB-M module, aroD-M module, aroE-M module | This study |
plasmid-3 | Plasmid pXMJ19-RiboJ-aroG MU-H derivate, containing aroB-L module, aroD-L module, aroE-L module | This study |
plasmid-4 | Plasmid pXMJ19-RiboJ-aroGMU-M derivate, containing aroB-H module, aroD-M module, aroE-L module | This study |
plasmid-5 | Plasmid pXMJ19-RiboJ-aroG MU-M derivate, containing aroB-M module, aroD-L module, aroE-H module | This study |
plasmid-6 | Plasmid pXMJ19-RiboJ-aroG MU-M derivate, containing aroB-L module, aroD-H module, aroE-M module | This study |
plasmid-7 | Plasmid pXMJ19-RiboJ-aroG MU-L derivate, containing aroB-H module, aroD-L module, aroE-M module | This study |
plasmid-8 | Plasmid pXMJ19-RiboJ-aroG MU-L derivate, containing aroB-M module, aroD-H module, aroE-L module | This study |
plasmid-9 | Plasmid pXMJ19-RiboJ-aroG MU-L derivate, containing aroB-L module, aroD-M module, aroE-H module | This study |
pXMJ19-GBTDE | Plasmid 2 derivate, containing a terminator between aroB and aroD Module | This study |
pXMJ19-GBTDTE | Plasmid pXMJ19-GBTDE derivate, containing a terminator between aroD and aroE module | This study |
pXMJ19-GTBTDTE | Plasmid pXMJ19-GBTDTE derivate, containing a terminator between aroG and aroB module | This study |