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Table 3 Overflow metabolism of acetate

From: Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics

Strain

YNB + Gluc

Acetate production

Acetate max @ time

Acetate re-uptake

TY757

 

0.977 ± 0.149

12.9 ± 2.2 @ 12 h

0.672 ± 0.125

TY920

 

0.918 ± 0.142

11.0 ± 1.6 @ 12 h

0.693 ± 0.209

TY952

 

1.261 ± 0.075

14.4 ± 0.4 @ 12 h

0.698 ± 0.019

TY985

 

1.742 ± 0.072

23.2 ± 1.3 @ 12 h

1.433 ± 0.150

TY1018

 

1.750 ± 0.118

20.2 ± 0.7 @ 12 h

1.018 ± 0.061

TY954

 

1.517 ± 0.270

12.2 ± 2.6 @ 8 h

0.417 ± 0.012

TY1040

 

0.754 ± 0.320

9.5 ± 0.3 @ 24 h

0.377 ± 0.145

TY1040

w/ Met

1.091 ± 0.111

10.7 ± 1.3 @ 12 h

0.276 ± 0.073

TY1032

w/ Met

2.239 ± 0.664

19.0 ± 6.2 @ 8 h

0.679 ± 0.154

TY1041

 

1.994 ± 0.694

15.1 ± 1.5 @ 12 h

0.224 ± 0.027

TY1041

w/ Met

1.578 ± 0.389

12.8 ± 0.9 @ 24 h

0.351 ± 0.215

TY1031

w/ Met

1.551 ± 0.209

13.5 ± 1.6 @ 8 h

0.403 ± 0.071

  1. All uptake/production rates in mmol/g DCW/h. Maximum detected values in mmol/g DCW