Figure 3

Impact of pH on organic acid production by A. brasiliensis BRFM1877 in glucose–nitrate-containing medium. Glucose (blue square), gluconic acid (black triangle), ethanol (blue circle), and lactic acid (orange circle) concentrations in cultures carried out in MM2 liquid medium started with 50 g/L of glucose then added with 20 g/L of glucose at 96 h of culture, without (A) and with (B) addition of 80 g/L CaCO₃ after 24 h of culture. The evolution of pH in both cultures, with (black triangle) and without (violet square) CaCO3 addition, is also presented (C). Experiments were carried out in triplicate. Error bars show standard deviations.