|
Plasmids
| | |
|
pACYCDuet
|
P15A ori, Cmr
|
Novagen
|
|
pCDFDuet
|
CloDE13 ori, Strr
|
Novagen
|
|
pETDuet
|
f1 ori, Ampr
|
Novagen
|
|
pGEX 5X-3
|
pBR322ori, Ampr
|
GE Healthcare
|
|
pA-SeTAL
|
pACYCDuet carrying TAL from Saccharothrix espanaensis
|
Kim et al. (2013) [26]
|
|
pA-aorG-SeTAL-tyrA
|
pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from E. coli
|
Kim et al. (2013) [26]
|
|
pA-aorGfbr-SeTAL-tyrAfbr
|
pACYCDuet carrying TAL from S. espanaensis, aroG
fbr
, and tyrA
fbr from E. coli
|
This study
|
|
pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr
|
pACYCDuet carrying TAL from S. espanaensis, aroG
fbr
, PPSA, tktA, and tyrA
fbr from E. coli
|
Kim et al. (2013) [26]
|
|
pE-pIbF6′H2-pOs4CL
|
pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by independent T7 promoter.
|
This study
|
|
pE-pIbF6′H2-Os4CL
|
pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by one T7 promoter.
|
This study
|
|
pG-pIbF6′H2-pOs4CL
|
pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. F6′H2 is controlled by pTac promoter and 4CL is controlled by T7 promoter.
|
This study
|
|
pG-pIbF6′H2-Os4CL
|
pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter.
|
This study
|
|
pG-pIbF6′H1-Os4CL
|
pGEX 5X-3 harboring F6′H1 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter.
|
This study
|
|
BL21 (DE3)
|
F-
ompT hsdS
B
(rB
- mB
-) gal dcm lon (DE3)
|
Novagen
|
|
BtyrR
|
BL21(DE3) ΔtyrR
|
Kim et al. (2013) [26]
|
|
BtyrR-tyrA
|
BL21(DE3) ΔtyrR/ΔtyrA
|
Kim et al. (2013) [26]
|
|
BydiI
|
BL21(DE3) ΔydiI
|
This study
|
|
BybgC
|
BL21(DE3) ΔybgC
|
This study
|
|
B-CM1
|
BL21 harboring pE-pIbF6′H2-Os4CL
|
This study
|
|
B-CM2
|
BL21 harboring pE-pIbF6′H2-pOs4CL
|
This study
|
|
B-CM3
|
BL21 harboring pG-pIbF6′H2-pOs4CL
|
This study
|
|
B-CM4
|
BL21 harboring pG-pIbF6′H2-Os4CL
|
This study
|
|
B-CM5
|
BL21 harboring pE-pIbF6′H1-Os4CL
| |
|
B-CM6
|
BL21 harboring pG-pIbF6′H2-Os4CL and pA-SeTAL
|
This study
|
|
B-CM7
|
BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA
|
This study
|
|
B-CM8
|
BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-SeTAL-tyrAfbr
|
This study
|
|
B-CM9
|
BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr
|
This study
|
|
B-CM10
|
BydiI harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA
|
This study
|
|
B-CM11
|
BybgC harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA
|
This study
|
|
B-CM12
|
BybgC harboring pG-pIbF6′H1-Os4CL, pA-aroGfbr-SeTAL-tyrAfbr, and pC-Sam5
|
This study
|
