Skip to main content

Table 1 Plasmids and strains used in the present study

From: Biological synthesis of coumarins in Escherichia coli

Plasmids or E. coli strain Relevant properties or genetic marker Source or reference
Plasmids   
pACYCDuet P15A ori, Cmr Novagen
pCDFDuet CloDE13 ori, Strr Novagen
pETDuet f1 ori, Ampr Novagen
pGEX 5X-3 pBR322ori, Ampr GE Healthcare
pA-SeTAL pACYCDuet carrying TAL from Saccharothrix espanaensis Kim et al. (2013) [26]
pA-aorG-SeTAL-tyrA pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from E. coli Kim et al. (2013) [26]
pA-aorGfbr-SeTAL-tyrAfbr pACYCDuet carrying TAL from S. espanaensis, aroG fbr , and tyrA fbr from E. coli This study
pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr pACYCDuet carrying TAL from S. espanaensis, aroG fbr , PPSA, tktA, and tyrA fbr from E. coli Kim et al. (2013) [26]
pE-pIbF6′H2-pOs4CL pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by independent T7 promoter. This study
pE-pIbF6′H2-Os4CL pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by one T7 promoter. This study
pG-pIbF6′H2-pOs4CL pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. F6′H2 is controlled by pTac promoter and 4CL is controlled by T7 promoter. This study
pG-pIbF6′H2-Os4CL pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter. This study
pG-pIbF6′H1-Os4CL pGEX 5X-3 harboring F6′H1 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter. This study
BL21 (DE3) F- ompT hsdS B (rB - mB -) gal dcm lon (DE3) Novagen
BtyrR BL21(DE3) ΔtyrR Kim et al. (2013) [26]
BtyrR-tyrA BL21(DE3) ΔtyrR/ΔtyrA Kim et al. (2013) [26]
BydiI BL21(DE3) ΔydiI This study
BybgC BL21(DE3) ΔybgC This study
B-CM1 BL21 harboring pE-pIbF6′H2-Os4CL This study
B-CM2 BL21 harboring pE-pIbF6′H2-pOs4CL This study
B-CM3 BL21 harboring pG-pIbF6′H2-pOs4CL This study
B-CM4 BL21 harboring pG-pIbF6′H2-Os4CL This study
B-CM5 BL21 harboring pE-pIbF6′H1-Os4CL  
B-CM6 BL21 harboring pG-pIbF6′H2-Os4CL and pA-SeTAL This study
B-CM7 BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA This study
B-CM8 BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-SeTAL-tyrAfbr This study
B-CM9 BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr This study
B-CM10 BydiI harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA This study
B-CM11 BybgC harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA This study
B-CM12 BybgC harboring pG-pIbF6′H1-Os4CL, pA-aroGfbr-SeTAL-tyrAfbr, and pC-Sam5 This study