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Table 1 Plasmids and strains used in the present study

From: Biological synthesis of coumarins in Escherichia coli

Plasmids or E. coli strain

Relevant properties or genetic marker

Source or reference

Plasmids

  

pACYCDuet

P15A ori, Cmr

Novagen

pCDFDuet

CloDE13 ori, Strr

Novagen

pETDuet

f1 ori, Ampr

Novagen

pGEX 5X-3

pBR322ori, Ampr

GE Healthcare

pA-SeTAL

pACYCDuet carrying TAL from Saccharothrix espanaensis

Kim et al. (2013) [26]

pA-aorG-SeTAL-tyrA

pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from E. coli

Kim et al. (2013) [26]

pA-aorGfbr-SeTAL-tyrAfbr

pACYCDuet carrying TAL from S. espanaensis, aroG fbr , and tyrA fbr from E. coli

This study

pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr

pACYCDuet carrying TAL from S. espanaensis, aroG fbr , PPSA, tktA, and tyrA fbr from E. coli

Kim et al. (2013) [26]

pE-pIbF6′H2-pOs4CL

pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by independent T7 promoter.

This study

pE-pIbF6′H2-Os4CL

pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by one T7 promoter.

This study

pG-pIbF6′H2-pOs4CL

pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. F6′H2 is controlled by pTac promoter and 4CL is controlled by T7 promoter.

This study

pG-pIbF6′H2-Os4CL

pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter.

This study

pG-pIbF6′H1-Os4CL

pGEX 5X-3 harboring F6′H1 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter.

This study

BL21 (DE3)

F- ompT hsdS B (rB - mB -) gal dcm lon (DE3)

Novagen

BtyrR

BL21(DE3) ΔtyrR

Kim et al. (2013) [26]

BtyrR-tyrA

BL21(DE3) ΔtyrR/ΔtyrA

Kim et al. (2013) [26]

BydiI

BL21(DE3) ΔydiI

This study

BybgC

BL21(DE3) ΔybgC

This study

B-CM1

BL21 harboring pE-pIbF6′H2-Os4CL

This study

B-CM2

BL21 harboring pE-pIbF6′H2-pOs4CL

This study

B-CM3

BL21 harboring pG-pIbF6′H2-pOs4CL

This study

B-CM4

BL21 harboring pG-pIbF6′H2-Os4CL

This study

B-CM5

BL21 harboring pE-pIbF6′H1-Os4CL

 

B-CM6

BL21 harboring pG-pIbF6′H2-Os4CL and pA-SeTAL

This study

B-CM7

BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA

This study

B-CM8

BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-SeTAL-tyrAfbr

This study

B-CM9

BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr

This study

B-CM10

BydiI harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA

This study

B-CM11

BybgC harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA

This study

B-CM12

BybgC harboring pG-pIbF6′H1-Os4CL, pA-aroGfbr-SeTAL-tyrAfbr, and pC-Sam5

This study