The effects of extracellular pH and of the transcriptional regulator PACI on the transcriptome of Trichoderma reesei

Table 2 Differential expression of genes encoding CAZy family enzymes (glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and auxiliary activities) in the ∆ pac1 strain

Gene ID	Name	CAZy family	CAZy annotation	Expression at pH6 vs. pH3	*Expression in the strain ∆pac1 vs. QM9414 at pH6*
47268	bgl3i	GH3	Candidate beta-glucosidase	pH6 > pH3	*QM9414 > ∆pacC*
122495		GH76	Candidate alpha-1,6-mannanase	pH6 > pH3	*QM9414 > ∆pacC*
79671		CE9	N-acetyl-glucosamine-6-phosphate deacetylase	pH6 > pH3	*QM9414 > ∆pacC*
76227	cel3e	GH3	Candidate beta-glucosidase		*QM9414 > ∆pacC*
82235		GH31	Candidate alpha-glucosidase		*QM9414 > ∆pacC*
123226		GH37	Candidate alpha,alpha-trehalase		*QM9414 > ∆pacC*
71532		GH71	Candidate alpha-1 3-glucanase		*QM9414 > ∆pacC*
53542		GH76	Candidate alpha-1,6-mannanase		*QM9414 > ∆pacC*
69123		GH76	Candidate alpha-1,6-mannanase		*∆pacC > QM9414*

Genes showing differential expression in comparison of the strain ∆pac1 and QM9414 (significant difference in expression, with p value < 0.01 and fold change log2 > 0.4, detected at both time points of the triplicate cultures). For comparison the differential expression of the genes at different pH in the parental strain QM9414 is shown. Gene ID number (JGI, http://genome.jgi-psf.org/Trire2/Trire2.home.html), common name and the CAZy family and activity of enzyme encoded by the gene are included in the Table.

ISSN: 1475-2859