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Table 2 List of developed yeast strains

From: Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production

Strain Genotype Plasmid description Source
BY4741 MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0.   Research Genetics
EG60 MATα, ura3, trp1, his3.   Erica Golemis
EG60-2 MATα, ura3, trp1, his3. YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
EG60-3 MATα, trp1, his3 PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3   This study
EG60-4 MATα, ura3, trp1, his3. pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
EG60-5 MATα, ura3, trp1, his3. pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 This study
EG60-6 MATα, ura3, trp1, his3. pUTDH3/Sf126-EYFP, 2 μ PTDH3-Sf126-EYFP URA3 This study
AM228 MATα his3, ura3, trp1, rox1 derived from EG60   This study
ΑΜ228-1 MATα his3, ura3, trp1, rox1 derived from EG60 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
ΑΜ228-2 MATα his3, ura3, trp1, rox1 derived from EG60 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM229 MATα his3, ura3, trp1, rox1, dos2 derived from AM228   This study
AM229-1 MATα his3, ura3, trp1, rox1, dos2 derived from AM228 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
AM229-2 MATα his3, ura3, trp1, rox1, dos2 derived from AM228 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM230 MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229   This study
AM230-1 MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
AM230-2 MATα his3, ura3, trp1, rox1, dos2, yer134c derived from AM229 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM231 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230   This study
AM231-1 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
AM231-2 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5 derived from AM230 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM233 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231   This study
AM233-1 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
AM233-2 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w derived from AM231 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM238 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233   This study
AM238-1 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 YEplac195-YB/I/E (PTDH3-crtYB-tCYC1, PTDH3-crtI-tCYC1, PTDH3-crtE-tCYC1, URA3, 2 μ) This study
AM238-2 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study
AM238-3 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3); pHTDH/CD-HMG2 (PTDH3-CD-HMG2-tCYC1, 2 μ, HIS3; pWTDH/SCLSmat (PTDH3-SCLS-tCYC1, 2 μ TRP1 This study
AM238-4 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 This study
AM238-5 MATα his3, ura3, trp1, rox1, dos2, yer134c, vba5, ynr063w, ygr259c derived from AM233 pUTDH3/Sf126-EYFP, 2 μ PTDH3-Sf126-EYFP URA3 This study
AM109-1 MATa/α, GALp-(K6R)HMG2::HOX2, ura3, his3, trp1, PTDH3-HMG2(K6R)X2-::leu2, PTDH3-HMG2(K6R)::HO1, ERG9/erg9, UBC7/ubc7, ssm4/SSM4, pho86/PHO86, derived from AM102 pUTDH3/HMG1-TM-EYFP, 2 μ PTDH3-HMG1-TM-EYFP URA3 This study
AM109-2 MATa/α, GALp-(K6R)HMG2::HOX2, ura3, his3, trp1, PTDH3-HMG2(K6R)X2-::leu2, PTDH3-HMG2(K6R)::HO1, ERG9/erg9, UBC7/ubc7, ssm4/SSM4, pho86/PHO86, derived from AM102 pUTDH/CLS-ERG20 (F96C) (PTDH3-CcCLS-ERG20 (F96C)-tCYC1, 2 μ, URA3) This study