Figure 7

Sclareol titers in engineered yeast strains expressing CLS-ERG20 (F96C). Wild type EG60, AM228, AM229, AM230, AM233 and AM238 cells expressing CLS-ERG20 (F96C) were grown in 5 ml cultures in a roller drum for 24 hours. Subsequently, 500 μl dodecane overlay was added to the media and further incubated for 48 hours. The dodecane phase was analyzed by GC-MS and sclareol was quantified by GC-FID.