Figure 10

Lipid droplet quantification in the engineered strains. (A) Lipid droplet accumulation in 5 days old culture of yeast visualized by Nile red staining; (B) Overlays of flow cytometry analysis in EG60 cells (left) with empty vector (green) or expressing CLS-ERG20 (F96C) (yellow), and AM238 cells (right) with empty vector (blue) or expressing CLS-ERG20 (F96C) (red); (C) Mean fluorescence values of strains carrying empty vector (blue) or expressing CLS-ERG20 (F96C) (orange). 50.000 cells were counted from each strain.