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Table 1 Strains used in this study

From: Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae

Strain

Genotype

Source or reference

BY4741

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

A gift from Prof. Zhao Z

BY4741-BS

BY4741 containing pYC230-B-SUC2 a

This study

BY4741-NS

BY4741 containing pYC230-N-SUC2 b

This study

BY4741-LS

BY4741 containing pYC230-L-SUC2 c

This study

BY4741-BPS

BY4741 containing pYC230-BP-BSUC2 d

This study

BY4741-NPS

BY4741 containing pYC230-NP-BSUC2

This study

BY4741-LPS

BY4741 containing pYC230-LP-BSUC2

This study

NCYC625

Wild type, diploid

CGMCC

NCYC625-BS

NCYC625 containing pYC230-BSUC2

This study

NCYC625-NS

NCYC625 containing pYC230-NSUC2

This study

NCYC625-LS

NCYC625 containing pYC230-LSUC2

This study

L610

Wild type, diploid

Yang et al.

L610α

Haploid, MATα

This study

L610α suc2Δ

L610α in which SUC2 gene was deleted

This study

BY4741 suc2Δ

BY4741 in which SUC2 gene was deleted

This study

LB11

Heterozygote: L610α × BY4741

This study

LB12

Heterozygote: L610α × BY4741 suc2Δ

This study

LB13

Heterozygote: L610α suc2Δ × BY4741

This study

LB14

Heterozygote: L610α suc2Δ × BY4741 suc2Δ

This study

  1. aB-SUC2 indicates the SUC2 gene of BY4741;
  2. bN indicates the strain NCYC625;
  3. cL indicates the strain L610;
  4. dBP indicates PGK1 promoter in the plasmid pYC230-B-SUC2 was replaced by the promoter of BY4741 SUC2.