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Figure 4 | Microbial Cell Factories

Figure 4

From: Invertase Suc2-mediated inulin catabolism is regulated at the transcript level in Saccharomyces cerevisiae

Figure 4

Effect of SNPs in the SUC2 promoter on SUC2 transcript level. (A) SUC2 mRNA levels in S. cerevisiae strains BY4741, NCYC625 and L610 were determined by qRT-PCR, in which the SUC2 transcription level in strain BY4741 was set as 1, while others in strains NCYC625 and L610 were compared with it and showed as relative data; (B) Sequence alignment of SUC2 promoters from BY4741 (BP), NCYC625 (NP) and L610 (LP) by CLUSTAL W program. Important transcription factor binding sites were boxed in red. Nucleotide substitutions among three strains were blacked. A novel AGGGG sequence and a new TATA box formed by two nucleotide substitutions in SUC2 promoter of stain L610 were boxed in green; (C) Biomass (dry cell weight, gray), ethanol production (grid), inulinase activity (diagonal) and invertase activity (blank) of strain BY4741 overexpressing SUC2 under the control of different strength promoters were measured after cultured in inulin medium at 30°C for 48 h. Abbreviation BPS, NPS and LPS indicated SUC2 expression under the control of the promoter from strain BY4741, NY4741 and L610 respectively, and BS indicated SUC2 version from strain BY4741 under the control of the constitutive PGK1 promoter. Error bars represent standard deviations from the mean of three biological samples.

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