Figure 3

Inulin-fermenting performances of weak-inulin-utilizing yeast strains overexpressed with SUC2. (A) Biomass (dry cell weight, gray), ethanol production (grid), inulinase activity (diagonal) and invertase activity (blank) of strains BY4741 overexpressing different SUC2 versions were assayed after cultured in inulin medium at 30°C for 48 h, in which BS, NS and LS indicated different SUC2 versions from strains BY4741, NCYC625 and L610 respectively and error bars represent standard deviations from the mean of three biological samples; (B) Analysis of the inulin hydrolysate in the culture supernatant by ion chromatography, in which the supernatant of the 0-h culture was used as a control and all strains were cultivated in inulin medium for 48 h. Abbreviation G, F, and S represent glucose, fructose, and sucrose, respectively; (C) Biomass (dry cell weight, gray), ethanol production (grid), inulinase activity (diagonal) and invertase activity (blank) of strains NCYC625 overexpressing different SUC2 versions were assayed, in which the culture condition, the indication of BS, NS and LS and the representation of error bars are the same as that of BY4741 recombinants.