Table 1 Gene cassettes
From: Engineering Saccharomyces pastorianus for the co-utilisation of xylose and cellulose from biomass
Plasmid | Primers | Starting plasmid | Insertion site | |
|---|---|---|---|---|
TEFbgl1* | bgl1_Rec1F | bgl1_Rec2R | pRS42H | Sal1 |
PGKbgl1* | bgl1_Rec1F | bgl1_Rec2R | pRS42H | Sal1 |
TEFcbh2* | cbh2_Rec1F | cbh2_Rec2R | pRS42H | Sal1 |
PGKegl2* | egl2_Rec1F | egl2_MidR | pRS42H | Sal1 |
egl2_MidF | egl2_Rec2R | |||
PGKxyl1 | xyl1F | xyl1R | pRS42H_PGKbgl1 | Sal1 |
PGKxdh1 | xdhF | xdhR | pRS42H_PGKbgl1 | Sal1 |
PGKxi | xiF, xiMidF | xiMidR, xiR | pRS42H_PGKbgl1 | Sal1 |
PGKxdh1GFP | xdhF, GFPF | xdh1GFPR, GFPR | pRS42H_PGKbgl1 | Sal1 |
TEFXKS1 | Psi_TEFF, xks_MidF | xks_MidR, Psi_CycR | pRSK42K | Psi1 |
PGKxyl1-PGKxdh1 | Psi_PGKF xr_MidF | xr_MidR Psi_CycR | pRS42H_PGKxdh1 | Psi1 |
PGKxyl1-TEFXKS1-PGKxdh1 | Acc_TEFF, xks_MidF | xks_MidR, AccR | pRS42H_PGKxyl1-PGKxdh1 | Acc65I |
PGKxyl1-TEFbgl1-PGKxdh1 | Acc_TEFF, bgl1_MidF | bgl1_MidR, AccR | pRS42H PGKxyl1-PGKxdh1 | Acc65I |
PGKxyl1-TEFcbh2-PGKxdh1 | Acc_TEFF, cbh2_MidF | cbh2_MidR, AccR | pRS42H PGKxyl1-PGKxdh1 | Acc65I |
PGKxyl1-PGKegl2-PGKxdh1 | Acc_PGKF, egl2_MidF | egl2_MidR, AccR | pRS42H PGKxyl1-PGKxdh1 | Acc65I |