Figure 5

Fermentation of pre-treated biomass liquor. Fermentations were carried out with S. pastorianus strains expressing the gene cassettes PGKxyl1-TEFXKS-PGKxdh1 in YEP media supplemented with spent grain liquor () or with purified sugars (xylose 30 g L⁻¹ and glucose 11 g L⁻¹) (). Fermentations were conducted at 30°C for 5 days and samples were taken at the intervals shown and assayed for A. Glucose (g L⁻¹), B. Xylose (g L⁻¹) and C. Alcohol (g L⁻¹). Control fermentations with S. pastorianus containing the empty vector pRSH42 in spent grain liquor () or with purified sugars () are also shown in C. Panel D; cell growth profile of S. pastorianus in SGL, and mixed sugar (xylose and glucose), at various times. Cells were inoculated at low cell density (1x10⁵ cell/mL). Mean values from triplicate experiments are shown and error bars indicate the standard error from the mean.