Figure 9
From: Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
Reactivity of ApoA-1 and Hsp70 in the Limulus amebocyte lysate (LAL) assay. ApoA-1 was produced in E. coli strain BW30270/pApo404 and its endotoxin-free derivatives KPM318/pApo404 and KPM335/pApo404, whereas Hsp70 was obtained from KPM404/pHsp70His and its parental strain BL21 (DE3)/pHsp70His. The proteins were minimally purified by IMAC and assayed with the LAL test. The measurements represent the means and standard deviations from three individual experiments.