Figure 8
From: Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
SDS-PAGE gels of ApoA-1 and Hsp70. The proteins were expressed in endotoxin-free derivatives of E. coli strains BW30270 (A) and BL21 (DE3) (B), respectively, and minimally purified using IMAC on HisTrap HP (1 ml) columns. The recombinant ApoA-1 and Hsp70 samples (6 μg each) were resolved under denaturing conditions using 12% and 10% polyacrylamide gels, respectively. Molecular mass protein markers (kDa) were run in lanes M. Arrows indicate the positions of ApoA-1 and Hsp70.