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Table 3 Primers used in this study

From: Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae

Target genes Primers
URA3 (fw) 5'- ttgttgttgctgctttgagttctttcttgtgtgagtgctacaagccacatttaaactaagtcaattacacaaagttagtgTTTTTTGTTCTTTTTTTTGA
URA3 (rv) 5'- cttagtttaaatgtggcttgGGGTAATAACTGATATAATTAAATTGAAGC
ILV1 (fw) 5'- AATTATATCAGTTATTACCCcaagccacatttaaactaagtcaattacacaaagttagtgaaccgacaatttactttataaatttacgcaacaacttgtt
ILV1 (rv) 5'- aatccttacgtctatgtttcaaaccttgttttcat