Figure 3
Time course of cultivation of and fermentation by YPH499ΔILV1 strain in isoleucine-containing media. (a) YPH499ΔILV1 was inoculated at an OD600 of 0.1 and cultured in SD minimal medium containing 0, 1.25, 3, 6, 12, 18, 24 or 30 mg/L isoleucine. Cell growth was determined by measuring OD600 using a spectrophotometer. (b)(c)(d)(e) YPH499ΔILV1 was inoculated at an OD600 of 2 and grown in SD minimal medium containing different concentrations of isoleucine. The cell growth was determined by measuring the OD600 using a spectrophotometer, and the concentrations of isobutanol, ethanol, and the total of 2-methyl-1-butanol and 3-methyl-1-butanol, in the media were determined using GC-MS. Each data point represents the mean (SD) values obtained from 3 replicate fermentations.