Time course of cultivation of and fermentation by
YPH499ΔILV1 strain in
isoleucine-containing media. (a) YPH499ΔILV1 was inoculated at an
OD600 of 0.1 and cultured in SD
minimal medium containing 0, 1.25, 3, 6, 12, 18, 24 or 30 mg/L
isoleucine. Cell growth was determined by measuring
OD600 using a spectrophotometer.
inoculated at an OD600 of 2 and grown in
SD minimal medium containing different concentrations of
isoleucine. The cell growth was determined by measuring the
OD600 using a spectrophotometer, and
the concentrations of isobutanol, ethanol, and the total of
2-methyl-1-butanol and 3-methyl-1-butanol, in the media were
determined using GC-MS. Each data point represents the mean (SD)
values obtained from 3 replicate fermentations.