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Figure 2 | Microbial Cell Factories

Figure 2

From: Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae

Figure 2

Isobutanol production by BY4741 single-gene knockout strains. BY4741-emp and ΔXXXX-emp are the control strains harboring the pATP426 empty vector. BY4741-kAI and ΔXXXX-kAI harbor the pATP426-kivd-ADH6-ILV2 plasmid for enhancing isobutanol biosynthesis. Cells were inoculated at an OD600 of 2 and grown in SD minimal or selectable media. For ΔILV1 strains, 60 mg/L of isoleucine was added to the SD medium. The concentration of isobutanol in the medium of each culture after 2 days of fermentation was determined using GC-MS. Each data point represents the mean (SD) values obtained from 3 replicate fermentations.

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Microbial Cell Factories

ISSN: 1475-2859

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