Figure 1

Schematic illustration of the MASH method. (A) S. cerevisiae cells were lysed with zymolyase at 37°C for 1 h to obtain protoplasts. (B) The protoplasts were subjected to low-speed centrifugation (2,500 × g) with 1.2 M sorbitol. (C) Crude mitochondria were obtained by high-speed centrifugation (20,000 × g) with 0.7 M sorbitol. Large circles stand for nuclei, cell debris, and unbroken cells. The small closed-circles stand for the crude mitochondria, the large open-circles stand for microsomes.