Figure 6

Ectoine production in fermentor. An initial reaction mixture containing 200 mM sodium aspartate, 200 mM glycerol, 100 mM KCl, 100 mM sodium phosphate buffer (pH7.0), and recombinant E. coli cells of 20 OD/mL was used. (A) Time profiles of aspartate consumption and ectoine excretion in fermentor. Legend: excreted ectoine (black squares), aspartate (white squares). After 12 h of bioconversion the feeding solution was added (↓) with a flow speed of 25 mL/h. (B) Repeated use of cells to synthesize ectoine in fermentor. Error bars respresent standard deviations from triplicate biological replicates.