Table 3 Primers and synthetized fragments
Oligonucleotides for pyrR gene deletion | |
|---|---|
Primer number | Sequence 5'- 3' |
pyrUP1 | AAAAGTGAGCGGATTGA |
pyrUP2 | TCCTGCCAGAGCATAGAG |
pyrDN1q | CTCTATGCTCTGGCAGGAGGGGTTTTTTCTTCAACAATCAGGGGGAAAT |
pyrDN2 | GGGCCCGGATCCCACTGTCACCCATAATAGAGC |
pyrG1 | GCATTCTTGTCGGCATTA |
pyrG2 | GCCACAGCAGGACTCATT |
Cat1qr | CATAAAAGCAGGTCTTCATCGCTCTATTATGTCTTCAACTAAAGCACCCAT |
Cat2pr | GGGCCCGGATCCTCTTCAACTAAAGCACCCAT |
araR1qr | CTGCCCCGTTAGTTGAAGGCATTTTCTGTCAATGTTTTC |
araR2qr | AATCCCTCTTGTCTTAATGCTTATTCATTCAGTTTTCGTG |
Oligonucleotides for pyrH gene overexpression | |
Primer number | Sequence 5'- 3' |
pyrHUP1 | TACGGCATTCACATCAGG |
pyrHUP2q | TCCACTTCATCCACTCCATCGCTTAACGCATTGATATGA |
P1h | ATGGAGTGGATGAAGTGGA |
P2h | CATTCTTTACCCTCTCCTTT |
pyrH1q | AAAGGAGAGGGTAAAGAATGGAAAAACCAAAATACAAACG |
pyrH2q | CCTTCTACACGATATGTGCTGCGTACAGTAGCCAATTCG |
pyrHDN1 | AGCACATATCGTGTAGAAGG |
pyrHDN2q | ATGGGTGCTTTAGTTGAAGAAATGGCTGTCGCTATTGTT |
Cat2h | GCTGTAATATAAAAACCTTC |
Cat1qh | AAGAAGAAGGCAATGACACGTCTTCAACTAAAGCACCCAT |
araR1qh | CTGCCCCGTTAGTTGAAGGCATTTTCTGTCAATGTTTTC |
araR2h | TTATTCATTCAGTTTTCGTG |
pyrHG1q | CACGAAAACTGAATGAATAATCAGCCTAATGATGTCTTGT |
pyrHG2 | ACTTCTTGAACGACTTCCA |
Promoter-h | ATGGAGTGGATGAAGTGGAATCGTTTTAGAATGGGAGAATTAACTATTAATGTTTGACAACTATTACAGAGTATGCTATAATAAATTCACAGAATAGTCTTTTAAGTAAGTCTACTCTGAATTTTTTTAAAAGGAGAGGGTAAAGA |
CX1h | ATGGAGTGGATGAAGTGGA |
CX2h | GCGTACAGTAGCCAATTCG |
Oligonucleotides for prs gene overexpression | |
Primer number | Sequence 5'- 3' |
prsUP1 | AATCCGCCGCTTCCAA |
prsUP2q | TGTCAAACATTAATAGTTAATTCTCCCATTCTAAAACGATTCCACTTCATCCACTCCAT |
p1p | CAAATGGAGTGGATGAAG |
p2p | ACTTCCCCGTCACTAAAT |
prs1 | TTAAAAGGAGAGGGTAAAGAATGTCTAATCAATACGGAGATAAG |
prs2 | TTAGCTGAACAGATAGCTGACT |
prsDN1q | AGTCAGCTATCTGTTCAGCTAATGTCCTCCATTGTGATTGAT |
prsDN2 | ACGCATGATGAAGAACTTG |
CR1qp | CCAAGTTCTTCATCATGCGTTCTTCAACTAAAGCACCCAT |
CR2p | TTATTCATTCAGTTTTCGTG |
prsG1q | CACGAAAACTGAATGAATAAATCAAGTGGCGGAAGAAG |
prsG2 | AGGATGCGATTCAATTATGC |
Promoter-p | CAAATGGAGTGGATGAAGTGGAATCGTTTTAGAATGGGAGAATTAACTATTAATGTTTGACAACTATTACAGAGTATGCTATAATAAATTCACAGAATAGTCTTTTAAGTAAGTCTACTCTGAATTTTTTTAAAAGGAGAGGGTAAAGAATGTCTAATCAATACGGAGATAAGAATTTAAAGATTTTTTCTTTGAATTCGAATCCAGAGCTTGCAAAAGAAATCGCATATATAGTTGGAGTTCAATTAGGGAAATGTTCTGTCACAAGATTTAGTGACGGGGAAGT |
CX1p | TCCGCCGATTACTTCTTG |
CX2p | CCATGTCACTATTGCTTCAG |
Oligonucleotides for hom gene deletion | |
Primer number | Sequence 5'- 3' |
homUP1 | TGAACTGACATTTGAACAT |
homUP2 | CCTTTCTTTTGATTGTCC |
homDN1q | CCAAGAGGACAATCAAAAGACGCTTTCTGCTGTTCATAA |
homDN2 | CGTGTCATTGCCTTCTTC |
Cat1qm | AAGAAGAAGGCAATGACACGTCTTCAACTAAAGCACCCAT |
Cat2m | GCTGTAATATAAAAACCTTC |
araR1qm | CTGCCCCGTTAGTTGAAGGCATTTTCTGTCAATGTTTTC |
araR2m | TTATTCATTCAGTTTTCGTG |
homG1q | CACGAAAACTGAATGAATAAAAGCGATTCGTGTAGGG |
homG2 | GATGGTCAGGAAGCAGT |
Oligonucleotides for nupC-pdp gene deletion | |
Primer number | Sequence 5'- 3' |
pnUP1 | GAAGTGTGCGAAGGATGT |
pnUP2 | GAGGAGAATGTAGCCAAGAA |
pnDN1q | GCAATTTATTCTTGGCTACATTCATTGGACGAAGCGAGAG |
pnDN2 | CTGCGGAGTTCCTTGTATC |
CR1qpn | ATGATACAAGGAACTCCGCAGTCTTCAACTAAAGCACCCAT |
CR2pn | TTATTCATTCAGTTTTCGTG |
pnG1q | CACGAAAACTGAATGAATAAATCTGCGATGTCAACTGTAT |
pnG2 | GGCTGCGTCTTCTTCTGTT |
Oligonucleotides for cdd gene deletion | |
Primer number | Sequence 5'- 3' |
cddUP1 | GCTTCAGGACGGACAGTTCAGT |
cddUP2q | AGATACTGGTCCAGGGGTGTCAGCACTTGGATTTGGAATACGGCG |
cddDN1 | GCTGACACCCCTGGACCAGTATCT |
cddDN2q | CGTTTGTTGAACTAATGGGTGCTTTCAGAACCGTTGTCCTGCCCTTT |
Cat1c | CACCCATTAGTTCAACAAACG |
Cat2c | TTCAACTAACGGGGCAG |
araR1qc | CTGCCCCGTTAGTTGAAGGCATTTTCTGTCAATGTTTTC |
araR2E | CGCGGATCCTGACACCCCTGGACCAGTATCT |
cddG1E | CGCGGATCCTGACACCCCTGGACCAGTATCT |
cddG2 | CCCACAATTCAAGGTAGACACG |
Oligonucleotides for pyrG gene constitutive expression and overexpression | |
Primer number | Sequence 5'- 3' |
pyrGUP1 | GGATACGGCGATGAAGGT |
pyrGUP2q | GTTCTCTCTTCGTTTTTGAAGAGCCCCCCAAAATACATACTACATAGTTCGAC |
pyrGDN1 | TTCAAAAACGAAGAGAGAACATAG |
pyrGDN2q | GTTTGTTGAACTAATGGGTGCTAATCGTTTGAGACAGGTTGC |
pyrGG1 | GTCCGCACGAAAACTGAATGAATACCAGCACGGTGAAGTATT |
pyrGG2 | CCAAGATCCTCAACATCCTT |
Cat1g | CACCCATTAGTTCAACAAACG |
Cat2g | TTCAACTAACGGGGCAG |
araR1qg | CTGCCCCGTTAGTTGAAGGCATTTTCTGTCAATGTTTTC |
araR2g | TTATTCATTCAGTTTTCGTG |
pyrGUP1b | TACGGCGATGAAGGTAAC |
pyrGUP2qb | AGCTCCCTTTCAATTTCTTGCGTACTATGTTCTCTCTTCGT |
gsiB1 | CAAGAAATTGAAAGGGAGCT |
gsiB2 | TTCCAAGTGAGGATACAACTCC |
pyrGDCRG1 | GGAGTTGTATCCTCACTTGG |
pyrGDCRG2 | CAATGATGCCGTCTGTTC |
Promoter-g | CAAGAAATTGAAAGGGAGCTATGTTTTTCTCAAATTGTAAGTTTATTTCATTGACAACTTTAAAAAGGATCGCTATAATAACCAATAAGGACAAAAGGAGGAATTCAAAATGACGAAATATATTTTTGTAACCGGGGGAGTTGTATCCTCACTTGGAA |
CX1g | ATGGAGTGGATGAAGTGGA |
CX2g | GCGTACAGTAGCCAATTCG |