Table 1 Pyrimidine and pyrimidine nucleoside produced by modifying different genes of the pyrimidine nucleotide biosynthesis pathway in B. subtilis after 72 h fermentation
Strain | Productivity (mg/L) | Ratio (%) | Productivity (mg/L) | Ratio (%) | Productivity (mg/L) | Ratio (%) | Molarity (mmol/L) |
|---|---|---|---|---|---|---|---|
Cytidine | Uridine | Uracil | Total | ||||
TD12 | 200.9 ± 8.3 | 14.9 ± 0.8 | 960.1 ± 39.1 | 9.5 | |||
TD12np | 260.3 ± 11.3 | 129.6 | 1187.5 ± 49.9 | 7970 | 508.2 ± 18.5 | 52.9 | 10.5 |
TD13 | 247.2 ± 9.6 | 440.7 ± 17.1 | 935.7 ± 31.9 | 11.2 | |||
TD13np | 326.9 ± 7.0 | 132.2 | 1571.4 ± 38.9 | 356.6 | 16.9 ± 0.1 | 1.8 | 7.9 |
TD33 | 1244.2 ± 53.9 | 558.6 ± 24.2 | 1696.7 ± 39.6 | 22.6 | |||
TD33np | 1423.0 ± 47.5 | 114.4 | 1684.6 ± 62.4 | 301.6 | 14.2 ± 0.2 | 0.8 | 22.9 |