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Table 2 Comparison of production of 3HB by E. coli AF1000 and PPA652ara by varied cultivation techniques

From: Cultivation strategies for production of (R)-3-hydroxybutyric acid from simultaneous consumption of glucose, xylose and arabinose by Escherichia coli

Process mode

Sugar

Strain

Time

CDW

Sugar consumed

3HB

HAc

mean q S total

mean q 3HB

Y 3HB/HAc

Y 3HB/CDW

Y 3HB/Sugar

   

(h)

(g L −1 )

(g L −1 )

(g L −1 )

(g L −1 )

(g g −1h −1 )

(mg g −1h −1 )

(g g −1 )

(g g −1 )

(g g −1 )

Batch

Glc

AF1000

5.5

4.18

8.90

0.51

0.96

1.0

68

0.54

0.12

0.06

Batch

Glc + Xyl + Ara

PPA652ara

7.3

4.88

9.65

0.31

0.00

0.7

25

→ ∞

0.06

0.03

N-depletion

Glc + Xyl + Ara

AF1000

16.0

0.27

2.73

0.64

0.48

0.2

29

1.33

2.37

0.23

N-depletion

Glc + Xyl + Ara

PPA652ara

15.0

0.39

3.79

0.54

0.54

0.3

31

1.00

1.38

0.14

N-fedbatch

Glc + Xyl + Ara

AF1000

11.0

10.37

37.05

1.84

2.66

0.6

45

0.69

0.18

0.05

N-fedbatch

Glc + Xyl + Ara

PPA652ara

11.0

11.82

36.75

1.87

0.77

0.6

38

2.43

0.16

0.05

  1. In the cases of nitrogen depletion and nitrogen-limited fed-batch, the values given in the table are calculated for the respective depleted/limited phase only, and do not include the batch phases.