Cultivation strategies for production of (R)-3-hydroxybutyric acid from simultaneous consumption of glucose, xylose and arabinose by Escherichia coli

Table 1 3HB production by assorted E. coli strain and plasmid combinations on varied sugar carbon sources

*E. coli* strain	Plasmid	Carbon source	IPTG	Growth rate	Final OD ₆₀₀	3HB	Y _3HB/OD
*E. coli* strain	Plasmid	Carbon source	(μM)	(h ⁻¹ )		(g L ⁻¹ )	(g L ⁻¹ OD ₆₀₀ ⁻¹ )
AF1000	pJBGT3RX	Glc	/	0.74	3.5	0.00	/
AF1000	pJBGT3RX	Glc	200	0.74	3.0	0.19	0.06
AF1000	pJBGRXT3	Glc	/	0.80	3.6	0.00	/
AF1000	pJBGRXT3	Glc	200	0.78	3.4	0.18	0.05
PPA652ara	pJBGT3RX	Glc	200	0.35	3.4	0.01	/
PPA652ara	pJBGT3RX	Xyl	200	0.46	4.4	0.18	0.04
PPA652ara	pJBGT3RX	Ara	200	0.45	3.2	0.17	0.05
PPA652ara	pJBGT3RX	Glc + Xyl + Ara	200	0.56	3.2	0.17	0.05

ISSN: 1475-2859