Skip to main content

Table 1 3HB production by assorted E. coli strain and plasmid combinations on varied sugar carbon sources

From: Cultivation strategies for production of (R)-3-hydroxybutyric acid from simultaneous consumption of glucose, xylose and arabinose by Escherichia coli

E. coli strain Plasmid Carbon source IPTG Growth rate Final OD 600 3HB Y 3HB/OD
(μM) (h −1 )   (g L −1 ) (g L −1 OD 600 −1 )
AF1000 pJBGT3RX Glc / 0.74 3.5 0.00 /
AF1000 pJBGT3RX Glc 200 0.74 3.0 0.19 0.06
AF1000 pJBGRXT3 Glc / 0.80 3.6 0.00 /
AF1000 pJBGRXT3 Glc 200 0.78 3.4 0.18 0.05
PPA652ara pJBGT3RX Glc 200 0.35 3.4 0.01 /
PPA652ara pJBGT3RX Xyl 200 0.46 4.4 0.18 0.04
PPA652ara pJBGT3RX Ara 200 0.45 3.2 0.17 0.05
PPA652ara pJBGT3RX Glc + Xyl + Ara 200 0.56 3.2 0.17 0.05
  1. OD600 = Optical density at 600 nm.