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Figure 1 | Microbial Cell Factories

Figure 1

From: Cultivation strategies for production of (R)-3-hydroxybutyric acid from simultaneous consumption of glucose, xylose and arabinose by Escherichia coli

Figure 1

3HB pathway introduced into E. coli by plasmid pJBG. Genes t3 (acetoacetyl-CoA thiolase) and rx (acetoacetyl-CoA reductase) were cloned from H. boliviensis into plasmid pJBG (pACYC184-derived) to produce pJBGT3RX (shown in figure by the gene order t3-rx) and pJBGRXT3 (gene order rx-t3). The plasmids were subsequently transformed into E. coli AF1000 and PPA652ara and used to produce 3HB in the medium. Conversion of 3HB-CoA into 3HB is likely catalyzed by the E. coli native enzyme thioesterase II (TesB).

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