Table 3 Plasmids used in this work
Plasmids | Genotype or description | Source |
|---|---|---|
pET28a | Vector for protein overproduction | Novagen |
pET28a-Acthi | pET28a derivative for ActhiS over-expression from E. coli | This study |
pYG013 | Vector for gene expression in A. chrysogenum | Zhang (2004) [60] |
pYG237 | pYG013 derivative for Acthi over-expression in A. chrysogenum | This study |
pYG239 | pYG013 derivative for silencing Acthi in A. chrysogenum | This study |