Table 2 Oligonucleotide primers used in this work
Primers for RT-qPCR | |
|---|---|
5’-Acthi-qPCR | 5’-CATGGGTGCCTTCTCCGTCA-3’ |
3’-Acthi-qPCR | 5’-GACCATGGCACCAAAGGTAGGT-3’ |
5’-AcveA | 5’-GTTCGAGGAGGGCAAGGAAATCA-3’ |
3’-AcveA | 5’-GGTCAAGATGGGAGGGTTGGGTT-3’ |
5’-AcsepH | 5’-GACAAAGAAGCGCAGGTGGTGG-3’ |
3’- AcsepH | 5’-GGGTTTACCTTGGAGCAGCTCGATA-3’ |
5’-cpcR1 | 5’-TGACGTCAAAGGAGCCAGTCGT-3’ |
3’-cpcR1 | 5’-GCGGCTGCGGAACAATGTAA-3’ |
5’-pcbAB | 5’-CGCTTGGCCGAGGAGAGAAA-3’ |
3’-pcbAB | 5’-ATGCATGCATCCAGCTGGCC-3’ |
5’-pcbC | 5’-AGGACATCCAGGCTGACGACACG-3’ |
3’-pcbC | 5’-CGCTCCTCGTTGACCCATTTGA-3’ |
5’-cefEF | 5’-CGCCGTTCTCAACTCTGTGGGC-3’ |
3’-cefEF | 5’-CGAGCGTGATGGTCGATAGGTCGTAG-3’ |
5’-cefG | 5’-GTTCGTATTCATCGCCAGGT-3’ |
3’-cefG | 5’-TATTCCGGCCGGCTTGGACT-3’ |
5’-AHAS | 5’-ATGCCACGTACAACGACATCGC-3’ |
3’-AHAS | 5’-CTTTGCGGCGTTGAACTCGG-3’ |
5’-PDC | 5’-TTGAGGATGTGACGCTTCGGG-3’ |
3’-PDC | 5’-TTGCCTACAAAGCCCTCCGC-3’ |
5’-TK | 5’- ATGGGCTACGGCGAGATTGACC-3’ |
3’-TK | 5’- TCCAGAGGACGTGGGCAATG-3’ |
5’actin-qPCR | 5’-GCCGCCCTCGTTATCGACAA -3’ |
3’actin-qPCR | 5’- CGTACGAGTCCTTCTGGCCCAT -3’ |
Primers for Acthi gene cloning and the construction of the pYG237 and pYG239 plasmids | |
Acthi-R1 | GCGGAATTCATGTCGCCTCCCGCTGCTAC |
Acthi-R2 | CCGTCTAGATCAGACCGCATTCTCCTTCT |
Acthi-sense-R3 | CGGAATTCGGCTTGAGCACCGCCTAC |
Acthi-sense-R4 | GGGAATTCCATATGGTTGGGGTCCATGCAGGA |
Acthi-antisense-R5 | CGTCTAGAGGCTTGAGCACCGCCTAC |
Acthi-antisense-R6 | ATCTCGAGGTTGGGGTCCATGCAGGA |
Intron-R7 | TGCTCACATATGTGAGCCTTTCTTCTTGCCTGTCA |
Intron-R8 | CGCTCGAGGTGTGTTTTGAACAATTTCACG |