Figure 7

Comparison of CPC production and transcriptional analysis of CPC biosynthetic genes in WT, Acthi(−) and Acthi(+). (A) CPC production of WT, Acthi(−) and Acthi(+). Error bars represent the standard deviations from three independent experiments. The procedures of fermentation and the detection CPC production were performed as described in the Methods. The CPC production of Acthi(+) was dramatically increased and had a 42.4% decrease in Acthi(−) compared with WT. (B) The expression of cephalosporin biosynthetic genes in WT, Acthi(−) and Acthi(+). The primers used for RT-qPCR are listed in Table 2. The relative abundance of the mRNAs was normalized against the actin gene levels. Error bars represent the standard deviations from three independent experiments. Significant differences in the relative expression levels of the cephalosporin biosynthetic genes in Acthi(−) and Acthi(+) compared with WT are indicated as “*”; p value confidence levels: “*” < 0.05, “**” < 0.01, and “***” < 0.001.