Figure 6

Esterase-catalysed desymmetrization of a prochiral malonic acid ester. Cell lysate from a cultivation Synechocystis sp. sfGFP-ST0071 cell lysate (500 μL of a 750 mL cultivation, OD 6.5) was added to a solution of 5 mM phenyl methyl malonate diethyl ester 1 and incubated for 48 h at 37°C. The reaction was stopped by addition of HCl (200 μL, 2 M), extracted twice with methyl tert-butyl ester. The optical purity of (S)-2 was determined using by chiral HPLC.