Figure 4

Synthesized CBA does not have inhibitors for isobutanol production. A. Isobutanol production from AL17(pAL603) using 20 g/L glucose (20gL Glu), 40 g/L glucose (40gL Glu), gluconate (GA) or synthesized CBA (CBA Syn). Glucose and gluconate were tested as growth substrates with or without one of the individual chemical components used in CBA synthesis (ABTS; 0.1 mM ABTS or Na Cit; 30 mM Na citrate). 20Glu&CBA Syn; 20 g/L glucose plus 40 g/L CBA Syn. B. Growth of AL17(pAL603) in all tested conditions. Where n = 3 biological replicates except for 20GLu& CBA Syn, n = 4, and error bars represent standard deviation.