Figure 2

Identification of the gene responsible for natural cellobionic acid metabolism in E. coli. Growth of strains with CBA (A) or glucose (B). AL17 (isobutanol host strain, Table 1) (circle), AL17 with ascB deleted (square), AL17∆ascB complemented with ascB expression from a plasmid (EascB) (diamond), AL17∆ascB expressing cellobionic acid phosphorylase (CBAP) (triangle). Where n = 3 biological replicates, and error bars represent standard deviation.