Table 1 Strains and plasmids used in this study

S. rimosus

M4018

a strain used for commercial production of OTC

[27]

ΔotcR

otcR gene disruption mutant, derived from M4018

This study

ΔotcR::otcR

otcR complemented strain, derived from ΔotcR

This study

M4018::otcR

otcR overexpressed strain, derived from M4018

This study

M4018/pST

Integrated a control plasmid pST in the genome of M4018

This study

M4018::SFotcR

Integrated a copy of SF14-driven otcR in the genome of M4018

This study

M4018::2SFotcR

Integrated two copies of SF14-driven otcR in the genome of M4018

This study

M4018::3SFotcR

Integrated three copies of SF14-driven otcR in the genome of M4018

This study

E.coli

TOP10

General cloning host for plasmid manipulation

Novagen

ET12567 (pUZ8002)

Donor strain for conjugation between E. coli and Streptomycetes

[38]

DH5α

Host for reporter system

Novagen

Plasmids

p4-16 h-7 s

Fosmid harbouring entire oxy gene cluster

This study

pOtcRDM

For deletion otcR gene, containing left and right arms of otcR

This study

pGusT-SF14

Derived from pSET152, containing SF14-driven gusA

[39]

pST

Derived from pGusT-SF14, deleting the SF14-driven gusA in pGusT-SF14

This study

pSF14-otcR

Derived from pGusT-SF14, SF14-driven otcR

This study

pSF142-otcR

Derived from pSF14-otcR, containing two copies of SF14-driven otcR

This study

pSF143-otcR

Derived from pSF142-otcR, containing three copies of SF14-driven otcR

This study

pOtcR

Derived from pGusT-SF14, containing a copy of otcR driven by its own promoter

This study

pTAC

Template plasmid containing gfp

[40]

pSF14-GFP

Derived from pGusT-SF14, containing SF14-driven gfp

This study

pAGFP

Derived from pSF14-GFP, inserting oxyA promoter into upstream of gfp

This study

pIGFP

Derived from pSF14-GFP, inserting oxyI promoter into upstream of gfp

This study

pI1*GFP

Derived from pIGFP, inserting oxyI1* promoter into upstream of gfp

This study

pI2*GFP

Derived from pIGFP, inserting oxyI2* promoter into upstream of gfp

This study

pJGFP

Derived from pSF14-GFP, inserting oxyJ promoter into upstream of gfp

This study

pRGFP

Derived from pSF14-GFP, inserting oxyR promoter into upstream of gfp

This study

pSGFP

Derived from pSF14-GFP, inserting oxyS promoter into upstream of gfp

This study

pRAGFP

Derived from pAGFP, inserting otcR driven by SF14

This study

pRIGFP

Derived from pIGFP, inserting otcR driven by SF14

This study

pRI1*GFP

Derived from pI1*GFP, inserting otcR driven by SF14

This study

pRI2*GFP

Derived from pI2*GFP, inserting otcR driven by SF14

This study

pRJGFP

Derived from pJGFP, inserting otcR driven by SF14

This study

pRRGFP

Derived from pRGFP, inserting otcR driven by SF14

This study

pRSGFP

Derived from pSGFP, inserting otcR driven by SF14

This study

pOtcR-AGFP

Derived from pAGFP, inserting otcR driven by its own promoter

This study

pOtcR-IGFP

Derived from pIGFP, inserting otcR driven by its own promoter

This study

pOtcR-JGFP

Derived from pJGFP, inserting otcR driven by its own promoter

This study

pOtcR-RGFP

Derived from pRGFP, inserting otcR driven by its own promoter

This study

pOtcR-SGFP

Derived from pSGFP, inserting otcR driven by its own promoter

This study