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Table 1 Strains and plasmids used in this study

From: Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus

Name Description Sources
S. rimosus   
M4018 a strain used for commercial production of OTC [27]
ΔotcR otcR gene disruption mutant, derived from M4018 This study
ΔotcR::otcR otcR complemented strain, derived from ΔotcR This study
M4018::otcR otcR overexpressed strain, derived from M4018 This study
M4018/pST Integrated a control plasmid pST in the genome of M4018 This study
M4018::SFotcR Integrated a copy of SF14-driven otcR in the genome of M4018 This study
M4018::2SFotcR Integrated two copies of SF14-driven otcR in the genome of M4018 This study
M4018::3SFotcR Integrated three copies of SF14-driven otcR in the genome of M4018 This study
E.coli   
TOP10 General cloning host for plasmid manipulation Novagen
ET12567 (pUZ8002) Donor strain for conjugation between E. coli and Streptomycetes [38]
DH5α Host for reporter system Novagen
Plasmids   
p4-16 h-7 s Fosmid harbouring entire oxy gene cluster This study
pOtcRDM For deletion otcR gene, containing left and right arms of otcR This study
pGusT-SF14 Derived from pSET152, containing SF14-driven gusA [39]
pST Derived from pGusT-SF14, deleting the SF14-driven gusA in pGusT-SF14 This study
pSF14-otcR Derived from pGusT-SF14, SF14-driven otcR This study
pSF142-otcR Derived from pSF14-otcR, containing two copies of SF14-driven otcR This study
pSF143-otcR Derived from pSF142-otcR, containing three copies of SF14-driven otcR This study
pOtcR Derived from pGusT-SF14, containing a copy of otcR driven by its own promoter This study
pTAC Template plasmid containing gfp [40]
pSF14-GFP Derived from pGusT-SF14, containing SF14-driven gfp This study
pAGFP Derived from pSF14-GFP, inserting oxyA promoter into upstream of gfp This study
pIGFP Derived from pSF14-GFP, inserting oxyI promoter into upstream of gfp This study
pI1*GFP Derived from pIGFP, inserting oxyI1* promoter into upstream of gfp This study
pI2*GFP Derived from pIGFP, inserting oxyI2* promoter into upstream of gfp This study
pJGFP Derived from pSF14-GFP, inserting oxyJ promoter into upstream of gfp This study
pRGFP Derived from pSF14-GFP, inserting oxyR promoter into upstream of gfp This study
pSGFP Derived from pSF14-GFP, inserting oxyS promoter into upstream of gfp This study
pRAGFP Derived from pAGFP, inserting otcR driven by SF14 This study
pRIGFP Derived from pIGFP, inserting otcR driven by SF14 This study
pRI1*GFP Derived from pI1*GFP, inserting otcR driven by SF14 This study
pRI2*GFP Derived from pI2*GFP, inserting otcR driven by SF14 This study
pRJGFP Derived from pJGFP, inserting otcR driven by SF14 This study
pRRGFP Derived from pRGFP, inserting otcR driven by SF14 This study
pRSGFP Derived from pSGFP, inserting otcR driven by SF14 This study
pOtcR-AGFP Derived from pAGFP, inserting otcR driven by its own promoter This study
pOtcR-IGFP Derived from pIGFP, inserting otcR driven by its own promoter This study
pOtcR-JGFP Derived from pJGFP, inserting otcR driven by its own promoter This study
pOtcR-RGFP Derived from pRGFP, inserting otcR driven by its own promoter This study
pOtcR-SGFP Derived from pSGFP, inserting otcR driven by its own promoter This study
  1. *The asterisk is used to distinguish the engineered promoter from the native promoter (PoxyI).