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Table 2 The primers used in this study

From: Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli

Primers

Sequence

Cel-CD-NcoI-Forward

5′-TTTTCCATGGAAGGAAACACTCGTGAAGA-3′

Cel-CD-XhoI-Reverse

5′-TTTTCTCGAGAAGTACTTTCGTGTATTTTG-3′

Cel-CD-BamHI-Reverse

5′-TTTTGGATCCAAGTACTTTCGTGTATTTTG-3′

Cel-20-BamHI-Reverse

5′-TTTTGGATCCGCGTTTAACATTGTCATTAC-3′

Cel-CD5-NcoI-Forward

5′-TTTTCCATGGAAGACAATTTTAAACATTT-3′

Cel-CD10-NcoI-Forward

5′-TTTTCCATGGAACATTTATTAGGTAATGACAA-3′

Cel-CD15-NcoI-Forward

5′-TTTTCCATGGACAATGTTAAACGCCCTTC-3′

Cel-CD20-NcoI-Forward

5′-TTTTCCATGGAACCTTCTGAGGCTGGCGCATT-3′

Cel-CD30-NcoI-Forward

5′-TTTTCCATGGAAGTCGATGGACAAATGACA-3′

NRN1-BamHI-Forward

5′-TTTTGGATCCGCTGGTAAATGTGACGCCG-3′

NRN1-XhoI-Reverse

5′-TTTTCTCGAGTTAACCATTGCCCGAGCCG-3′

MBP-BamHI-Forward

5′-TTTTGGATCCATCGAAGAAGGTAAACTGG-3′

MBP-XhoI-Reverse

5′-TTTTCTCGAGTTACTTGGTGATACGAGTC-3′

GlpQ–BamHI-Forward

5′-TTTTGGATCCGCGGACAGCAACGAAAAAA-3′

GlpQ-XhoI-Reverse

5′-TTTTCTCGAGTTACTCTTTATTAAGAAATT-3′

CBD–BamHI-Forward

5′-TTTTGGATCCGACCAGGTCAGCGTCCGCTT-3′

CBD-XhoI- Reverse

5′-TTTTCTCGAGTTATGGCTGCCAATTCACGT-3′

pelC–BamHI-Forward

5′-TTTTGGATCCGCAGATAAGGTTGTCCACGA-3′

pelC-XhoI- Reverse

5′-TTTTCTCGAGTTAGAACTGGGTGTTATTAT-3′

Glu160Gln-Forward

5′-TTATTTATGAGTTAGCGAATCAGCCAAGTAGTA-3′

Glu160Gln-Reverse

5′- GATTCGCTAACTCATAAATAATGTGTGGATTGT-3′

Glu274Gln-Forward

5′-GAGTAGCGGTATTTGCGACACAATGGGGAACGA-3′

Glu274Gln-Reverse

5′-GTGTCGCAAATACCGCTACTCCGTTTTCTAACG-3′