Figure 4From: Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli The expression and secretion of Cel-CD and its N-terminal nested-deletion mutants. Cells were harvested 16 h after induction and washed with 1 mL of 100 mM MOPS (pH 7.0). The whole cells (1), the supernatant of the disrupted cells (2) and the released protein in the medium (3) were separated on 12% SDS-PAGE.Back to article page