Figure 2

Subcellular localization and accumulation process analysis of Cel-CD. (A) Subcellular localization of Cel-CD. Cells were harvested 4 and 16 h after induction and washed with 1 mL of 100 mM MOPS (pH 7.0), then subjected to cold osmotic shock to separate the periplasm from spheroplasts. (B) The accumulation process of Cel-CD at different locations. Cells were harvested after 8 h induction and washed with 1 mL of 100 mM MOPS (pH 7.0), then suspended in 50 mL of fresh LB containing 80 μg/mL chloramphenicol. The quantification of Cel-CD was performed by Bio-Rad Quantity One Version 4.2.1. Lanes are marked as: S, spheroplasts; P, periplasm; H, hypertonic solution; M, medium.