Figure 1From: Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli Secretion analysis of Cel-CD in E. coli BL21 (DE3). SDS-PAGE analysis and activity assay of the Cel-CD in the culture medium. Samples were collected by centrifugation at the indicated times after induction. The endoglucanase activity of the secreted Cel-CD was determined by Congo red staining as described in the Materials and Methods. Molecular size markers are shown in kDa. Lane M is the marker; Lane C is the control of collected cells.Back to article page