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Figure 4 | Microbial Cell Factories

Figure 4

From: Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae

Figure 4

Reaction profiles of hydroxylation of fatty acids by FoCYP539A7 and FoCYP655C2 with the heterologous ( ScCPR ) reductase. ΔPox1 mutant S. cerevisiae cells harboring the CYP39A7-ScCPR and CYP655C2-ScCPR reconstituted systems were induced with 4% galactose, 2 mM 5-ALA and 500 μM of substrates: caprylic acid (C8), capric acid (C10) and lauric acid (C12) were added and cultured at pH 7.0. Samples collected at 10 hr intervals were extracted, trimethylsilyl derivatized and analyzed by GC.

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