Figure 3From: Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae Schematic representation of the strategy used to disrupt pox1 gene of S. cerevisiae INV S c1 by PCR-mediated short-region homologous recombination. The HisMX cassette was used to replace the pox1gene. The double alleles of pox1 are replaced by the HisMX auxotrophic marker through homologous recombinationBack to article page