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Figure 4 | Microbial Cell Factories

Figure 4

From: Improved cell surface display of Salmonella enterica serovar Enteritidis antigens in Escherichia coli

Figure 4

SDS-PAGE analysis of recombinant Salmonella antigens recovered from E. coli outer membrane fractions. Lane 1: Size marker (Spectra Multicolour Broadrange, Fermentas); Lane 2: O17ΔOmpT/pAIDA1-SefA (positive control; shake flask cultivation from a prior experiment [7]; Lane 3: empty O17ΔOmpT (negative control); Lane 4: non-induced O17ΔOmpT/pAIDA1-SefA (negative control); Lane 5: O17ΔOmpT/pAIDA1-H:gmd; Lane 6: O17ΔOmpT/pAIDA1-H:gmdSefA; Lane 7 & 8: O17ΔOmpT/pAIDA1-SefA, replicates.

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