Figure 3
Flow cytometry histograms of various Salmonella antigens expressed on the surface of E. coli . The presence of the His6-tag and c-Myc-tag in the displayed proteins were probed with THE™ His Tag Antibody [FITC] and Anti-c-Myc [SureLight® Allophycocyanin] antibody, respectively. Negative controls (cells lacking surface display plasmids) are all shown in red. (A) Left panel: His-probed E. coli/pAIDA1-H:gm (light blue) and E. coli/pAIDA1-SefA (green). Right panel: c-Myc-probed E. coli/pAIDA1-H:gm (light blue) and E. coli/pAIDA1-SefA (green). (B) Left panel: His-probed E. coli/pAIDA1-H:gmd (dark blue), E. coli/pAIDA1-SefA (green), and E. coli/pAIDA1-H:gmdSefA (purple). Right panel: c-Myc-probed E. coli/pAIDA1-H:gmd (dark blue), E. coli/pAIDA1-SefA (green), and E. coli/pAIDA1-H:gmdSefA (purple).