An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture

Table 1 REST 2009 output for all genes assayed using qRT-PCR

Gene	Type	Expression	Std. Error	95% C.I.	P(H1)	Result
gyrB	REF	1
hoxF	TRG	4.6	3.663 - 5.539	2.945 - 6.720	0	UP
hypF2	TRG	2.5	1.407 - 3.677	0.938 - 5.930	0.035	UP
hoxA	TRG	4.4	3.814 - 4.959	3.527 - 5.467	0.002	UP

95% C.I. represents 95% confidence intervals for expression ratios. P(H1) is the p-value that indicates the significance of the observed regulation. Result represents the significance of up- or down-regulation of the target genes. gyrB was used as an endogenous reference gene. REF represents a reference gene, whereas TRG – a target gene.

ISSN: 1475-2859