Figure 2From: Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris Treatment of purified proRCLCNQ and r27RCLC by glycosidase. A. SDS-PAGE analyses of purified proRCLCNQ and r27RCLC treated with PNGase F. B. Western blotting verification of proRCLCNQ and r27RCLC treated with PNGase F. Lane M, Marker; Lane 1, proRCLCNQ; Lane 2, proRCLCNQ treated with PNGase F; Lane 3, r27RCLC; Lane 4, r27RCLC treated with PNGase F.Back to article page