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Table 3 S. cerevisiae strains used in this study

From: Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae

Name

Genotype

Notes

Origin

CEN.PK113-5D

MATa; ura3-52; MAL2-8C; SUC2

Haploid MATa lab strain with uracil auxotrophy

Euroscarf

CEN.PK113-7D

MATa; MAL2-8C; SUC2

Prototrophic haploid MATa lab strain

Euroscarf

S01

CEN.PK2-1c, bar1Δ

BAR1 gene deleted

This study

S02

CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1

BAR1 and FUS1 deleted, Argonaute gene integrated at TRP1

This study

S03

CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1

RNAi capable base strain with Argonaute and Dicer integration

This study

GFP01

CEN.PK113-5D, bar1::phleo, fus1::KanMX, ura3-52::PTEF1-GFPCLN2PEST-ADH1t-pRS406

constitutive destabilized GFP expression

This study

GFP02

CEN.PK113-5D, ura3::PMAL12-GFPCLN2PEST-ADH1t-pRS406

MAL12 regulated GFP expression

This study

GFP03

CEN.PK113-5D, ura3::PGLC3-GFPCLN2PEST-ADH1t-pRS406

GLC3 regulated GFP expression

This study

GFP04

CEN.PK113-5D, ura3::PGPH1-GFPCLN2PEST-ADH1t-pRS406

GPH1 regulated GFP expression

This study

GFP05

CEN.PK113-5D, ura3::PSUC2-GFPCLN2PEST-ADH1t-pRS406

SUC2 regulated GFP expression

This study

GFP06

CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, pRS413

RNAi capable, constitutive GFP expressing control strain

This study

GFP07

CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, PSUC2-GFPantisense-CYC1t-pRS413

SUC2 promoter mediated repression of GFP using RNAi

This study