Skip to main content

Table 3 S. cerevisiae strains used in this study

From: Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae

Name Genotype Notes Origin
CEN.PK113-5D MATa; ura3-52; MAL2-8C; SUC2 Haploid MATa lab strain with uracil auxotrophy Euroscarf
CEN.PK113-7D MATa; MAL2-8C; SUC2 Prototrophic haploid MATa lab strain Euroscarf
S01 CEN.PK2-1c, bar1Δ BAR1 gene deleted This study
S02 CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1 BAR1 and FUS1 deleted, Argonaute gene integrated at TRP1 This study
S03 CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1 RNAi capable base strain with Argonaute and Dicer integration This study
GFP01 CEN.PK113-5D, bar1::phleo, fus1::KanMX, ura3-52::PTEF1-GFPCLN2PEST-ADH1t-pRS406 constitutive destabilized GFP expression This study
GFP02 CEN.PK113-5D, ura3::PMAL12-GFPCLN2PEST-ADH1t-pRS406 MAL12 regulated GFP expression This study
GFP03 CEN.PK113-5D, ura3::PGLC3-GFPCLN2PEST-ADH1t-pRS406 GLC3 regulated GFP expression This study
GFP04 CEN.PK113-5D, ura3::PGPH1-GFPCLN2PEST-ADH1t-pRS406 GPH1 regulated GFP expression This study
GFP05 CEN.PK113-5D, ura3::PSUC2-GFPCLN2PEST-ADH1t-pRS406 SUC2 regulated GFP expression This study
GFP06 CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, pRS413 RNAi capable, constitutive GFP expressing control strain This study
GFP07 CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, PSUC2-GFPantisense-CYC1t-pRS413 SUC2 promoter mediated repression of GFP using RNAi This study