Name | Genotype | Notes | Origin |
---|---|---|---|
CEN.PK113-5D | MATa; ura3-52; MAL2-8C; SUC2 | Haploid MATa lab strain with uracil auxotrophy | Euroscarf |
CEN.PK113-7D | MATa; MAL2-8C; SUC2 | Prototrophic haploid MATa lab strain | Euroscarf |
S01 | CEN.PK2-1c, bar1Δ | BAR1 gene deleted | This study |
S02 | CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1 | BAR1 and FUS1 deleted, Argonaute gene integrated at TRP1 | This study |
S03 | CEN.PK2-1c, bar1Δ, fus1::KanMX, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1 | RNAi capable base strain with Argonaute and Dicer integration | This study |
GFP01 | CEN.PK113-5D, bar1::phleo, fus1::KanMX, ura3-52::PTEF1-GFPCLN2PEST-ADH1t-pRS406 | constitutive destabilized GFP expression | This study |
GFP02 | CEN.PK113-5D, ura3::PMAL12-GFPCLN2PEST-ADH1t-pRS406 | MAL12 regulated GFP expression | This study |
GFP03 | CEN.PK113-5D, ura3::PGLC3-GFPCLN2PEST-ADH1t-pRS406 | GLC3 regulated GFP expression | This study |
GFP04 | CEN.PK113-5D, ura3::PGPH1-GFPCLN2PEST-ADH1t-pRS406 | GPH1 regulated GFP expression | This study |
GFP05 | CEN.PK113-5D, ura3::PSUC2-GFPCLN2PEST-ADH1t-pRS406 | SUC2 regulated GFP expression | This study |
GFP06 | CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, pRS413 | RNAi capable, constitutive GFP expressing control strain | This study |
GFP07 | CEN.PK2-1c, bar1Δ, fus1::KanMX, ura3:: PTEF1-yEGFPCLN2PEST-406, trp1::pRS404-PTEF-Ago1, leu2::pRS405-PTEF-Dcr1, PSUC2-GFPantisense-CYC1t-pRS413 | SUC2 promoter mediated repression of GFP using RNAi | This study |